摘要
目的 找出龟甲胶、鹿角胶、阿胶、黄明胶、新阿胶5种胶类药材的特征肽段,从而建立专属性的鉴别方法。方法 用胰蛋白酶针对样品进行酶解,采用液质联用多肽识别技术和数据处理软件对蛋白质酶解后的胶类样品(主含多肽混合物)进行分析处理,进行特征肽段的寻找;采用串联四级杆质谱的多反应检测(multiple reaction monitor, MRM)建立专属性鉴别方法。结果 找出了5种胶类药材的特征离子,确定阿胶的特征离子为m/z 539.8,鹿角胶的特征离子为m/z 765.4,龟甲胶的特征离子为m/z 631.3,黄明胶的特征离子为m/z 641.3,新阿胶的特征离子为m/z774.5,并建立了专属性强的鉴别方法。结论 确定的各胶类的特征肽专属性强,建立各胶类的专属性鉴别方法可以有效地区分各胶类。
Abstract
OBJECTIVE To find the marker peptides of five kinds of glue medicines including deerhorn glue, tortoise shell glue, donkey-hide gelatin,bovine-hide gelatin, and pig-hide gelatin. METHODS The characteristic peptides of different gelatins were identified by tripsin digestion and HPLC-MS/MS. RESULTS The marker peptides of five glue medicines were found, ie, donkey-hide gelatin m/z 539.8, deerhorn glue m/z 765.4, tortoise shell glue m/z 631.3, bovine-hide gelatin m/z 641.3, pig-hide gelatin m/z 774.5, and the METHODS which can be used to identify the glue medicines were established according to the marker peptides. CONCLUSION The marker peptides that we found can be used for the identification of glue medicines.
关键词
胶类药材 /
特征肽段 /
液质联用技术 /
主成分分析 /
质量控制 /
质量标准
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Key words
glue medicine /
marker peptide /
LC-MS /
PCA /
quality control /
quality standard
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程显隆,陈佳,李明华,张倩倩,刘薇,魏锋*,马双成*.
特征肽段检测技术用于胶类药材专属性鉴别方法研究[J]. 中国药学杂志, 2015, 50(2): 104-108 https://doi.org/10.11669/cpj.2015.02.003
CHENG Xian-long, CHEN Jia, LI Ming-hua, ZHANG Qian-qian, LIU Wei, WEI Feng*, MA Shuang-cheng*.
Identification Study of Glue Medicines[J]. Chinese Pharmaceutical Journal, 2015, 50(2): 104-108 https://doi.org/10.11669/cpj.2015.02.003
中图分类号：
R284
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参考文献
[1]Ch. P(2010) VolⅠ(中国药典2010年版. 一部). 2010: 175-176.
[2]Ch. P(2010) VolⅠ(中国药典2010年版. 一部). 2010:302-303.
[3]Ch. P(2010) VolⅠ(中国药典2010年版. 一部). 2010:169.
[4]CHENG X L, LI W J, WEI F,et al. Advances in studies on identificated method of gelatins. Asia-Pacific Tradit Med(亚太传统医药),2011,7(3):167-169.
[5]WANG B,XIAO X Y,CHENG X L, et al. Study on relative molecular mass distribution of protein and peptide in donkey hide. Chin J Pharm Anal(药物分析杂志),2009,29(11):1886-1891.
[6]CHENG X L, WEI F, XIAO X Y, et al. Identification of five gelatins by ultra performance liquid chromatography/time- of- flight mass spectrometry (UPLC/Q-TOF-MS) using principal component analysis . J Pharm Biomed Anal,2012,62(1):191-195.
[7]CHENG X L, WEI F, ChEN J, et al. Using the doubly charged selected ion coupled with MS/MS fragments monitoring (DCSI-MS/MS) mode for the identification of gelatin species. J Analy Meth Chem,2014, Article ID 764397, doi:10.1155/2014/764397.
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脚注
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基金
国家十二五“重大新药创制”专项(2014ZX09304307-002);国家自然科学基金青年项目(81202909)
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